BPI Digital Week Webinar - Multi-Mode Mimetic Ligand Library
Multi-mode chromatography has come to prominence in recent years due to the general utility of multi-mode ligands for product capture and polishing. However, significant optimisation of binding and elution conditions is often required to achieve the desired level of purity. To overcome the deficiencies of individual multi-mode ligands, we have developed a diverse library of Multi-Mode Mimetic Ligands™ which incorporate both ionic and hydrophobic groups, which offer significant flexibility in use. The Multi-Mode Mimetic Ligand™ Library is organised into a 12 x 8 array micro-column block and comprises a diverse array of ligands with a broad spread of charge, hydrophobic and hydrophilic functionalities. The adsorbents are produced using an agarose support matrix and incorporate triazine attachment chemistry for ease of synthesis and superior stability, Screening can be performed in high-throughput mode using robotic liquid handling systems or by use of conventional hand-held multi-channel pipettes. This approach allows for rapid screening, and identification of potential ligands for a specific target. Furthermore, adjustment of the starting protein fraction to suit individual ligands is not required as the diversity of the library enables the identification of binding ligands which are best-suited to the existing conditions and impurities. This approach has been evaluated in a variety of applications, including development of ligands for isolation of specific proteins from complex mixtures and the removal of specific contaminants.