Please enter your details to access this Video

First Name
Last Name
Company Name
*By completing and submitting this form, you understand and agree that use of Prometic’s web site is subject to Prometic’s Privacy Policy including the fact that Prometic may transfer your personal information collected in connection with your registration on this website to its affiliates globally and to third party entities that provide services to Prometic. The data you have provided will be used for Prometic Life Sciences Inc. to send you technical updates, special offers and product related information from Prometic Bioseparations.
Thank you!
Error - something went wrong!

LabRoots 2019 - Flow Through Polishing and The Use of Multi Mode Ligand Libraries to Improve Process

February 1, 2019 Buzz Lobbezoo
Multi-mode chromatography has come to prominence in recent years due to the general utility of multi-mode ligands for product capture and polishing. In particular, their application in flow-through polishing has significantly improved process economy. However, significant optimisation of binding and elution conditions is often required to achieve the desired level of purity. To overcome the deficiencies of individual multi-mode ligands, we have developed a diverse library of Multi-Mode Mimetic Ligands™ incorporating both ionic and hydrophobic groups, which offer significant flexibility in use. The Multi-Mode Mimetic Ligand™ Library is organised into a 12 x 8 array micro-column block and comprises a diverse array of ligands with a broad spread of charge, hydrophobic and hydrophilic functionalities. The adsorbents are produced using an agarose support matrix and incorporate triazine attachment chemistry for ease of synthesis and superior stability, Screening can be performed in high-throughput mode using robotic liquid handling systems or by use of conventional hand-held multi-channel pipettes. This approach allows for rapid screening, and identification of potential ligands for a specific target. Furthermore, adjustment of the starting protein fraction to suit individual ligands is not required as the diversity of the library enables the identification of binding ligands which are best-suited to the existing conditions and impurities. This approach has been evaluated in a variety of applications, including development of ligands for the removal of specific contaminants in a flow through mode. Learning Objectives: 1. Understand how you can use a diverse library of synthetic ligands to screen for adsorbents for your molecules that are difficult to purify using standard adsorbents. The screening library is fast becoming a must-have technology in the tool-box of a process development scientist. 2. A case study of using the Multi-Mode Mimetic Ligand™ Library to deliver a flow-through polishing step to remove FXI from human blood plasma.

No Previous Videos

Next Video
BPI Digital Week Webinar - Multi-Mode Mimetic Ligand Library
BPI Digital Week Webinar - Multi-Mode Mimetic Ligand Library

Multi-mode chromatography has come to prominence in recent years due to the general utility of multi-mode l...